Title : Hepato-renal protective potential of dimethyl fumarate in alloxan- induced diabetic mice model
Abstract:
Diabetes mellitus is a growing epidemic and is the most common cause of chronic kidney disease and liver failure. Despite advances in diabetes-related treatments, the effects of the disease have not yet been adequately reversed or prevented in patients. Therefore, there is an urgent need to develop more effective medication-assisted treatments. In this study, forty female mice were divided into eight groups with five animals in each. Type 2 diabetes mice models was established using multiple low-dose alloxan, and the diabetic mice were treated with three doses of dimethyl fumarate (DMF) i.e low, medium, and high viz. 20, 40 and 80 mg/kg, respectively for a period of 21 days. Then, specific test were done to evaluate blood biochemical parameters, oxidative stress markers, inflammatory cytokine, and histopathological changes in mice kidney and liver.Diabetic liver and kidney tissue showed marked dilation of bile ducts, tubules, infiltration, and inflammation. In addition, nuclear factor erythroid-2 related factor 2 (Nrf2) and its downstream effectors (Sirt1) were suppressed in these two diabetic tissues (p< 0.001). On the contrary, diabetic mice treated with DMF showed improvement in oxidative stress indices, inflammation, and this with increased Nrf2 and Sirt1 activation (p< 0.001). Collectively, it can be concluded that the role of DMF as a blood sugar reducer and improvement of diabetes complications is very significant, likely via reducing activation of pro-inflammatory cytokines function. Although, some contradictory effects of inflammatory factors were observed in kidney and liver tissues, but overall, the therapeutic combination of DMF and metformin (MET) has a good hepato-renal protective ability in alloxan-induced diabetic mice model.
Audience Take Away Notes:
- Audience can improve his knowledge about animal model of diabetes.
- Role of dimethyl fumarate in preventing of diabetes in experimental model of diabetes.
- In clinical pharmacology and toxicology can help to use some co-treatments in control of diabetes.
- With accurate study about molecular mechanisms of dimethyl fumarate we can reach to more anti-inflammatory signaling for better control of diabetes.
