Speaker at International Conference and Expo on Toxicology and Applied Pharmacology Conference  2022 - Izharul Haq
Indian Institute of Technology Guwahati , India
Title : Bioremediation of Petroleum Refinery Wastewater Using Bacillus Subtilis IH-1 and Assessment of Toxicity


With the rapid expansion of the population and the modernization of civilization, environmental contamination from the petroleum refinery sector has surged, necessitating immediate repair.Petroleum pollutinh-degrading bacteria are common in nature and may use these molecules as carbon and energy sources. The microbial bioremediation of petroleum-related pollutuion. In this sttudy, a bacterial culture was obtained from petroleum-contaminated sludge in order to valorize PAHs and biodegrade petroleum waste water samples. Bacillus subtilis IH-1 was discovered as the bacterial strain after it was examined. After 6 days, the bacteria had degraded 20.3% naphthalene and 25.9% phenanthrene. The treatment of waste water samples was evaluated using physicochemical and Fourier-transform infrared spectroscopy (FTIR) analysis, which revealed that the level of pollutants was high and exceeded the allowable limits. However, after bacterial degradation there was a significant reduction in pollution parametres. The phytoxicity test using v. mungo revealed a concentration-dependent decrease in seed germination, root length, shoot length, and biomass when compared to the control. The mitotic index of A. cepa root tips planted in varied concentrations of petroleum refinery wastewater samples was significantly lower than thatof the control root tip exposed to tap water, indicating the induction of cytotoxicity. However, after treatment with a bacterial strain, both phytotoxicity and cytotoxicity dramatically decreased, indicating that the bacterium has the capacity to breakdown the harmful contamination of petroleum wastewater and might be employed in large-scale studies.


Dr. Izharul Haq worked as a Post-Doctoral Fellow in the Department of Civil Engineering, Indian Institute of Technology Guwahati, India. He has obtained his PhD in Microbiology from CSIR-Indian Institute of Toxicology Research, Lucknow, India. He is working on the theme of liquid and solid waste management through microorganisms and their toxicity evaluation.He has been honored with the prestigious award “Young Scientist Award 2018” to recognize his scientific research. Dr. Haq has published more than twenty research and review papers in reputed national and international journals with high impact factors. He has published many book chapters in splendid international books and has also published four books with reputed international publishers. He has supervised four M Tech students in the Environmental Engineering field. He is appointed as Review Editor on the Editorial Board of Water and Wastewater Management, Frontiers in Environmental Science. He also serves as a reviewer in many international journals published by different publishers.